Protein Engineering

D-Amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217

Microbiology / Gene expression / Multidisciplinary / Protein Engineering / Sequence alignment / Escherichia coli / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / Lipoprotein(a) / Enzyme / Introns / Amino Acid Profile / Molecular Mass / Genome sequence / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Molecular Sequence Data / Escherichia coli / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / Lipoprotein(a) / Enzyme / Introns / Amino Acid Profile / Molecular Mass / Genome sequence / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Molecular Sequence Data

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Using Co-Cultures Expressing Fluorescence Resonance Energy Transfer Based Protein Biosensors to Simultaneously Image Caspase3 and Ca 2+ Signaling

Engineering / Technology / Calcium / Apoptosis / Protein Engineering / Fluorescence Resonance Energy Transfer / Biological Sciences / Caspases / Cercopithecus aethiops / Animals / Cell Death / Calcium Signaling / Fluorescence Imaging / Caspase / Hydrogen Peroxide / Fluorescent Protein / Biosensing Techniques / Fluorescence Resonance Energy Transfer / Biological Sciences / Caspases / Cercopithecus aethiops / Animals / Cell Death / Calcium Signaling / Fluorescence Imaging / Caspase / Hydrogen Peroxide / Fluorescent Protein / Biosensing Techniques

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HBV core particles as a carrier for B cell/T cell epitopes. Intervirology. 2001;44(2-3):98-114

Molecular virology / Protein Engineering / Genetic Engineering

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HBV Core Particles as a Carrier for B Cell/T Cell Epitopes

Microbiology / Medical Microbiology / Gene Therapy / Molecular virology / Self Assembly / Protein Engineering / Influenza virus / Genetic Engineering / High Level Synthesis / Hepatitis B / Humans / Animals / Cytomegalovirus / Knowledge base / Fine Structure Constant / Amino Acid Profile / Molecular Conformation / Simian Immunodeficiency Virus / Amino Acid Sequence / Hepatitis B virus / Heterologous Expression / Experimental Data / X Ray Crystallography / Epitopes / Molecular Sequence Data / Protein Engineering / Influenza virus / Genetic Engineering / High Level Synthesis / Hepatitis B / Humans / Animals / Cytomegalovirus / Knowledge base / Fine Structure Constant / Amino Acid Profile / Molecular Conformation / Simian Immunodeficiency Virus / Amino Acid Sequence / Hepatitis B virus / Heterologous Expression / Experimental Data / X Ray Crystallography / Epitopes / Molecular Sequence Data

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A recombinant transductor–effector system: In vitro study of G inhibitory protein (G-alpha-i1) direct activators

Protein Engineering / Saccharomyces cerevisiae / Escherichia coli / G protein / In Vitro Studies / Recombinant Proteins / Biochemistry and cell biology / Recombinant Proteins / Biochemistry and cell biology

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A recombinant transductor–effector system: In vitro study of G inhibitory protein (G-alpha-i1) direct activators

Protein Engineering / Saccharomyces cerevisiae / Escherichia coli / G protein / In Vitro Studies / Recombinant Proteins / Biochemistry and cell biology / Recombinant Proteins / Biochemistry and cell biology

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A recombinant transductor–effector system: In vitro study of G inhibitory protein (G-alpha-i1) direct activators

Protein Engineering / Saccharomyces cerevisiae / Escherichia coli / G protein / In Vitro Studies / Recombinant Proteins / Biochemistry and cell biology / Recombinant Proteins / Biochemistry and cell biology

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Water-based nanoparticulate polymeric system for protein delivery

Water / Vaccines / Biotechnology / Nanotechnology / Polymers / Multidisciplinary / Protein Engineering / Permeability / Mice / Female / Animals / Drug Delivery Systems / Rats / Buffers / Particle Size / Chickens / Time Factors / Surface Properties / Freeze Drying / Sensitivity and Specificity / Drug Stability / Hydrogen-Ion Concentration / Multidisciplinary / Protein Engineering / Permeability / Mice / Female / Animals / Drug Delivery Systems / Rats / Buffers / Particle Size / Chickens / Time Factors / Surface Properties / Freeze Drying / Sensitivity and Specificity / Drug Stability / Hydrogen-Ion Concentration

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High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins

Chemical Engineering / Inorganic Chemistry / Inorganic / Protein Engineering / Copper / Pseudomonas aeruginosa / Pseudomonas Aeruginosa / Site-directed Mutagenesis / Oxidation-Reduction / Pseudomonas aeruginosa / Pseudomonas Aeruginosa / Site-directed Mutagenesis / Oxidation-Reduction

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Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design

Kinetics / Biotechnology / Industrial Biotechnology / Gene expression / Protein Engineering / Environmental Biotechnology / Biocatalysis / Protein Design / Mutation / Escherichia coli / Medical Biotechnology / Temperature / Hydrogen Bonding / Theoretical Models / Active site / Protein Secondary Structure Prediction / Buffers / Substrate Specificity / Bioreactors / Phosphates / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Protein Binding / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Oxidation-Reduction / Environmental Biotechnology / Biocatalysis / Protein Design / Mutation / Escherichia coli / Medical Biotechnology / Temperature / Hydrogen Bonding / Theoretical Models / Active site / Protein Secondary Structure Prediction / Buffers / Substrate Specificity / Bioreactors / Phosphates / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Protein Binding / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Oxidation-Reduction

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Adding Diverse Noncanonical Backbones to Rosetta: Enabling Peptidomimetic Design

Algorithms / Computational Biology / Multidisciplinary / Protein Engineering / Software / Peptidomimetics / PLoS one / Peptidomimetics / PLoS one

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Lactobacillli expressing llama VHH fragments neutralise Lactococcus phages

Technology / Flow Cytometry / Bacteriophages / Scanning Electron Microscopy / Protein Engineering / Biological Sciences / Lactic Acid Bacteria / Genetic Enhancement / Animals / Lactobacillus / Molecular cloning / Recombinant Proteins / Cell Survival / Biological Sciences / Lactic Acid Bacteria / Genetic Enhancement / Animals / Lactobacillus / Molecular cloning / Recombinant Proteins / Cell Survival

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Specific Amyloid β Clearance by a Catalytic Antibody Construct

Biological Chemistry / Innate immunity / Gene expression / Protein Engineering / Biological Sciences / Brain / Humans / Mice / Animals / CHEMICAL SCIENCES / Recombinant Proteins / Hydrolysis / Alzheimer Disease / Brain / Humans / Mice / Animals / CHEMICAL SCIENCES / Recombinant Proteins / Hydrolysis / Alzheimer Disease

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De Novo Design of a Redox-Active Minimal Rubredoxin Mimic

Protein Engineering / Circular Dichroism / Zinc / The / Cobalt / Tryptophan / CHEMICAL SCIENCES / Protein Secondary Structure Prediction / Biomimetic materials / Cysteine / Oxidation-Reduction / Ferric Compounds / De Novo Design / Tryptophan / CHEMICAL SCIENCES / Protein Secondary Structure Prediction / Biomimetic materials / Cysteine / Oxidation-Reduction / Ferric Compounds / De Novo Design

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Molecular biomimetics: nanotechnology through biology

Molecular Biology / Biomimetics / Self Assembly / Crystallization / Nanotechnology / Multidisciplinary / Protein Engineering / Genetic Engineering / Molecular Recognition / Phage display / Proteins / Biomimetic materials / Control Structure / Inorganic chemicals / Soft Tissue / Amino Acid Sequence / Nature Materials / Protein Binding / Biocompatible Materials / Cell Surface Markers / Multidisciplinary / Protein Engineering / Genetic Engineering / Molecular Recognition / Phage display / Proteins / Biomimetic materials / Control Structure / Inorganic chemicals / Soft Tissue / Amino Acid Sequence / Nature Materials / Protein Binding / Biocompatible Materials / Cell Surface Markers

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Cementomimetics—constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides

Tissue Engineering / Protein Engineering / Humans / Peptides / Amelogenin / Biomimetic materials / DENTAL CEMENTUM / Calcium Binding Proteins / Tooth Calcification / Oral Science / Biomimetic materials / DENTAL CEMENTUM / Calcium Binding Proteins / Tooth Calcification / Oral Science

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